bee venom induces unfolded protein response in a172 glioblastoma cell line

نویسندگان

ali bazi cancer molecular pathology research center, faculty of medicine, imam reza hospital, mashhad university of medical sciences, mashhad, ir iran; faculty of allied medical sciences, zabol university of medical sciences, zabol, ir iran; faculty of allied medical sciences, zabol university of medical sciences, zabol, ir iran. tel/fax: +98-5432232166

mehran gholamin division of human genetics, immunology research center, avicenna research institute, mashhad university of medical sciences, mashhad, ir iran

mohsen sisakht department of biochemistry, faculty of medicine, mashhad university of medical sciences, mashhad, ir iran

mohammad reza keramati cancer molecular pathology research center, faculty of medicine, imam reza hospital, mashhad university of medical sciences, mashhad, ir iran

چکیده

background glioblastoma is a type of brain tumor with poor response to available therapies, and shows high rate of mortality. despite remarkable advancements in our knowledge about cytogenetic and pathophysiologic features of glioblastoma, current treatment strategies are mainly based on cytotoxic drugs; however, these therapeutic approaches are facing progressive failure because of the resistant nature of glioblastomas. in the recent years, however, promising results have emerged owing to targeted therapies toward molecular pathways within cancerous cells. unfolded protein response (upr) is a remarkable signaling pathway that triggers both apoptosis and survival pathways within cells, and therefore induces upr-related apoptotic pathways in cancer cells by er stress inducers. objectives recently, the role of bee venom (bv), which contains powerful bioactive peptides, in inducing upr-related apoptosis was revealed in cancer cell lines. nevertheless, currently there are no reports of bv potential ability in induction of upr apoptotic routes in glioblastoma. the aim of current study was to evaluate possible role of bee venome in inducing of upr pathway within a172 glioblastoma cell line. materials and methods we treated the a172 glioblastoma cell line with different bv doses, and assessed upr-related genes expression by real-time polymerase chain reaction (pcr). results the ic50 of bv for the studied cell line was 28 μg/ml. furthermore, we observed that bv can induce upr target genes (grp94 and gadd153) over-expression through a dose-dependent mechanism. conclusions our results suggest the potential role of bv as a therapeutic agent for glioblastomas.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Role of Oxidative Stress in Modulating Unfolded Protein Response Activity in Chronic Myeloid Leukemia Cell Line

Background: Recently, it has been revealed that tyrosine kinase inhibitors (TKIs) act through inducing both oxidative and endoplasmic reticulum (ER) stress in chronic myeloid leukemia cells. However, ER stress signaling triggers both apoptotic and survival processes within cells. Nevertheless, mechanisms by which TKIs avoid the pro-survival effects are not clear. The aim of this study was to ev...

متن کامل

Heat-induced apoptosis in human glioblastoma cell line A172.

OBJECTIVE Hyperthermia has been clinically applied to some types of brain tumors. However, the detailed mechanisms of this growth inhibition are not clear. The effect of mild hyperthermia on cultured human glioblastoma cell line, A172, was studied. METHODS A172 cells were heat treated (43-44.5 degrees C) for 1 hour in the growing phase. Cell viability was assessed by trypan blue dye exclusion...

متن کامل

role of oxidative stress in modulating unfolded protein response activity in chronic myeloid leukemia cell line

background: recently, it has been revealed that tyrosine kinase inhibitors (tkis) act through inducing both oxidative and endoplasmic reticulum (er) stress in chronic myeloid leukemia cells. however, er stress signaling triggers both apoptotic and survival processes within cells. nevertheless, mechanisms by which tkis avoid the pro-survival effects are not clear. the aim of this study was to ev...

متن کامل

Immunomodulatory Effects of Bee Venom in Human Synovial Fibroblast Cell Line

As in Iranian traditional medicine, bee venom (BV) is a promising treatment for the rheumatoid arthritis (RA) which is considered as a problematic human chronic inflammatory disease in the present time. Smoking is considered to be a major risk factor in RA onset and severity. The main aim of this study is to investigate the effects of BV on cigarette smoke-induced inflammatory response in fibro...

متن کامل

Immunomodulatory Effects of Bee Venom in Human Synovial Fibroblast Cell Line

As in Iranian traditional medicine, bee venom (BV) is a promising treatment for the rheumatoid arthritis (RA) which is considered as a problematic human chronic inflammatory disease in the present time. Smoking is considered to be a major risk factor in RA onset and severity. The main aim of this study is to investigate the effects of BV on cigarette smoke-induced inflammatory response in fibro...

متن کامل

Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172

  Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA br...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of biotechnology and health sciences

جلد ۲، شماره ۲، صفحات ۰-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023